By Allen I. Laskin (Eds.)
Meant for researchers in utilized microbiology and environmental engineers, this ebook covers such issues as environmental review of biotechnological procedures and microbial adjustments of haloaromatic and haloaliphatic compounds.
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Extra info for Advances in Applied Microbiology, Vol. 31
1976)that extracts ofC. acetobutylicum contain a NADH:ferredoxin oxidoreductase that both oxidizes NADH and reduces NAD, the former reaction being strongly activated by acetyl-CoA and the latter reaction being inhibited by NADH very much like the situation in C. , 1973). The NADPH: ferredoxin oxidoreductase apparently works only to produce NADPH for biosynthesis. As shown in Fig. 8, there is a high net flow ofelectrons from both NADH and pyruvate through ferredoxin to molecular hydrogen during acid- 32 PALMER ROGERS ogenesis, whereas during solvent formation extra electrons flow into the NADH pool to the more reduced end products such as butanol and ethanol.
Themsaccharolyticum (Avgerinos, 1982), C. , 1984), C. strain HIO-C. , 1983), C. saccharolyticum-unknown cellulolytic anaerobe (Murray and Khan, 1982a). In the first two well-defined cases the coculture produces a substantially higher yield of ethanol from cellulose than the monoculture of C. thermocellum. A metabolic explanation for the phenomenon of a stable coculture between C. thermocellum and C . thermohydrosulfuricum fermenting cellulose has been proposed (Ng and Zeikus, 1982). Glucose and cellobiose are produced from degradation of cellulose by cellulases excreted by C.
Acetobutylicum protoplasts by DNA from a kanamycin-resistant plasmid, pUB110, from Staphylococcus. Transformation to kanamycin resistance (KmR) required PEG 6000 and a preheating of the protoplasts at 55°C for 15 minutes to inactivate DNase. The plasmid, pUB110, was isolated from the KmR transformants. Mixtures of protoplasts from two double auxotroph strains of C . acetobutylicum have been induced to fuse using PEG for 3 to 15 minutes. , 1985). Electric field-mediated fusion has not been reported for clostridia (Zimmerman, 1983).